Understanding LiP Promoters from Phanerochaete chrysosporium: A Bioinformatic Analysis
نویسندگان
چکیده
Sergio Lobos1, Rubén Polanco2, Mario Tello3, Dan Cullen4, Daniela Seelenfreund1 and Rafael Vicuña5 1Laboratorio de Bioquímica, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile 2Escuela de Bioquímica, Facultad de Ciencias Biológicas, Universidad Andrés Bello 3 Centro de Biotecnología Acuícola, Universidad de Santiago de Chile. 4USDA Forest Service, Forest Products Laboratory, Madison, WI 53726, 5 Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Millennium Institute for Fundamental and Applied Biology 1,2,3,5Chile 4USA
منابع مشابه
Genomic organization of lignin peroxidase genes of Phanerochaete chrysosporium.
Three lignin peroxidase (LiP) genes from the basidiomycete Phanerochaete chrysosporium were cloned on a single 30 kb cosmid insert. One gene, GLG5, is the genomic equivalent of a previously reported cDNA clone, CLG5. The other two LiP genes are transcriptionally convergent and map to a position approximately 15 kb downstream of GLG5. The translational stop codons of these genes are separated by...
متن کاملExpression of lip genes during growth in soil and oxidation of anthracene by Phanerochaete chrysosporium.
mRNA extraction from soil and quantitation by competitive reverse transcription-PCR were combined to study the expression of the 10 known lignin peroxidase (lip) genes in anthracene-transforming soil cultures of Phanerochaete chrysosporium. Levels of extractable lipA transcript and protein (LiP H8) were well correlated, although they were separated by a 2-day lag period. The patterns of transcr...
متن کاملTemporal expression of the major lignin peroxidase genes of Phanerochaete chrysosporium.
DNA probes specific for the genes encoding major lignin peroxidase (LIP) isozymes H2, H8, and H10 of Phanerochaete chrysosporium were constructed. These probes were used to study the temporal expression of the three lip genes in defined low-nitrogen medium. H2 gene transcripts were produced at high levels on days 4, 5, and 7 and at low levels on day 6, while the H8 gene transcripts peaked on da...
متن کاملBiodegradation of 2,4-dichlorophenol in a fluidized bed reactor with immobilized Phanerochaete chrysosporium.
The performance of a fluidized bed reactor using immobilized Phanerochaete chrysosporium to remove 2,4-dichlorophenol (2,4-DCP) from aqueous solution was investigated. The contribution of lignin peroxidase (LiP) and manganese peroxidase (MnP) secreted by Phanerochaete chrysosporium to the 2,4-DCP degradation was examined. Results showed that Lip and Mnp were not essential to 2,4-DCP degradation...
متن کاملBiological Removal of Dibenzothiophene from Soil and Changes to soil Sulfate by White-Rot Fungus Phanerochaete chrysosporium
This study investigated biodegradation of dibenzothiophene (DBT) in marsh soil spiked bywhite-rot fungus Phanerochaete chrysosporium. Soil samples were spiked with 100 ppm DBTand incubated at 30°C in a dark chamber for 30 days. Samples were evaluated for pH, Mnperoxidaseactivity, sulfate ion concentration and growth during the tests. Results showedmaximum levels of pH, Mn-peroxidase and sulfate...
متن کامل